Wharton's Jelly Derived-Mesenchymal Stem Cells: Isolation and Characterization

  • Hossein Ranjbaran Immunogenetics Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Saeid Abediankenari Mail Immunogenetics Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Masoud Mohammadi Immunogenetics Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Narjes Jafari Department of Biostatistics and Community Medicine, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Alireza Khalilian Department of Biostatistics and Community Medicine, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Zahra Rahmani Department of Obstetrics, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Marzei Momeninezhad Amiri School of Traditional Medicine, Iran University of Medical Sciences, Tehran, Iran.
  • Pirooz Ebrahimi School of Traditional Medicine, Iran University of Medical Sciences, Tehran, Iran. AND Department of Molecular Medicine, Sapienza University, Rome, Italy.
Mesenchymal stem cells, Wharton`s jelly, Flow cytometry, Immunophenotyping


Wharton`s jelly-derived mesenchymal stem cells (WJ-MSCs), have a high proliferation valency and they do not produce teratogen or carcinogen after subsequent transplantation. They are known as regenerative medicine. Thus more research is needed on the isolation and characterization of mesenchymal stem cells. In this experimental study, we obtained Wharton's jelly tissues from mothers during normal vaginal delivery, after obtaining their informed consent. Mesenchymal stem cells were isolated from cultured Wharton`s jelly, cultured, and were then examined for their proliferation, immunophenotypes, and differentiation capacities. The immunophenotypes of WJ-MSCs were analyzed by flow cytometry. Differentiation was performed resulting in osteogenic, chondrogenic and adipogenic cells. WJ-MSCs formed a homogenous monolayer of adherent spindle-shaped cells. Our results showed the high capacity of the proliferation of WJ-MSCs. Immunophenotyping further confirmed the purity of the isolated cells; their surface antigen expression showed the phenotypical properties like those of WJ-MSCs. The expanded cells were positive for CD 90, CD105, and CD44; they were negative for CD34 and HLA-DR surface markers. The cells had the adipocytic, osteocytic and chondrogenic differentiation capacity. The isolation and characterization of WJ-MSCs with high purity had been conducted, and the results were obtained in a short span. The present study has revealed the feasibility of the culture medium with high glucose and 15% FBS in isolation and proliferation of WJ-MSCs. When Wharton`s jelly pieces were put in the dry bottom of the flask, very effective separation of the MSCs was achieved.


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How to Cite
Ranjbaran H, Abediankenari S, Mohammadi M, Jafari N, Khalilian A, Rahmani Z, Momeninezhad Amiri M, Ebrahimi P. Wharton’s Jelly Derived-Mesenchymal Stem Cells: Isolation and Characterization. Acta Med Iran. 56(1):28-33.