Preptin and Myostatin Independently Increase in Pre-Diabetics and Patients of Type 2 Diabetes Mellitus

  • Saeed Nazari Soltan Ahmad Department of Biochemistry, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Sajjad Nourollahi Department of Laboratory Medicine, School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Manouchehr Nakhjavani Endocrinology and Metabolism Research Center, Vali-Asr Hospital, Tehran University of Medical Sciences, Tehran, Iran.
  • Mehran Khojastehfard Department of Biochemistry, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  • Mostafa Mostafazadeh Department of Biochemistry, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Hamed Hajipour Department of Reproductive Biology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Davoud Sanajou Mail Department of Biochemistry, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Myostatin, Preptin, Insulin, HOMA-IR, Type 2 diabetes


The relation between serum preptin, myostatin, insulin, and also homeostatic model assessment-insulin resistance were examined in pre-diabetes persons and newly diagnosed patients with overt type 2 diabetes mellitus. A total of 84 subjects were included in the study and assigned into three groups: normoglycemic participants (group 1=27), pre-diabetes (group 2=30), and T2DM (group 3=29). Serum insulin, preptin, and myostatin levels were measured with immunoradiometric assay (IRMA), enzyme-linked immunosorbent assay (ELISA), and chemiluminescent immunoassay (CLIA), respectively. Patients with T2DM had higher levels of preptin compared to normoglycemic (461.25±53.90 vs. 407.54±54.78, P<0.001). Furthermore, these patients had elevated levels of myostatin compared with controls (2710.60±559.09 vs. 2246.37±416.40, P<0.001). Preptin and myostatin both positively correlated with serum insulin (r=0.369, P=0.01, and r=0.309, P=0.04, respectively). However, no significant association was found between serum preptin and myostatin levels. Stepwise multiple regression analysis showed that insulin was affected more by preptin, with only a trivial contribution from myostatin. Serum preptin and myostatin levels increase in pre-diabetic subjects and even further in type 2 diabetic patients. The correlation between preptin and insulin evolves when pre-diabetes or overt type 2 diabetes develops. Moreover, serum myostatin increases in association with insulin and not HOMA-IR in diabetic conditions.


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How to Cite
Nazari Soltan Ahmad S, Nourollahi S, Nakhjavani M, Khojastehfard M, Mostafazadeh M, Hajipour H, Sanajou D. Preptin and Myostatin Independently Increase in Pre-Diabetics and Patients of Type 2 Diabetes Mellitus. Acta Med Iran. 57(3):160-166.