Investigation of mRNA Expression Levels of TGIFLX and OCT1 Homeobox Genes in Colorectal Cancer
-
Narges Sadeghipour
,
Farnaz Lotfiyani
,
Reza Raoofian
,
Mohammad-Reza Noori-Daloii
,
Cyrus Azimi
,
Mansour Heidari
Abstract
The OCT1 and TGIFLX transcription factors are members of homeodomains whose expressions have been implicated in normal and abnormal development. However, the expression of TGIFLX and OCT1 in colorectal cancer is unknown. This study aimed to detect the expression of OCT1 and TGIFLX in clinical samples of colorectal cancer. Twenty-six pairs of colorectal cancer tissue and adjacent non-tumoral tissue were obtained at the time of surgery from patients with colorectal cancer. The expression of TGIFLX and OCT1 was detected by real time reverse transcriptase polymerase chain reaction (RT-PCR). OCT1 was down-regulated in colorectal carcinoma samples in both males (58.33%) and females (57.14%). By contrast, TGIFLX was mainly (41.63%) expressed in colorectal tumors of males' samples but not in para-neoplastic normal tissues. OCT1 expression was not significantly associated with the gender and site of primary tumor (P>0.05), but the expression of TGIFLX was associated with male patients (P<0.05). In conclusion, dysregulation of OCT1 and TGIFLX genes might be novel prognostic biomarkers for patients with colorectal cancer.
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8. Mark M, Rijli FM, Chambon P. Homeobox genes in embryogenesis and pathogenesis. Pediatr Res 1997;42:421-9.
9. Gehring WJ, Hiromi Y. Homeotic genes and the homeobox. Annu Rev Genet 1986;20:147-73.
10. Holland PW, Booth HA, Bruford EA. Classification and nomenclature of all human homeobox genes. BMC Biol 2007;5:47.
11. Gehring, W.J., M. Affolter, and T. Burglin, Homeodomain proteins. Annu Rev Biochem 1994;63:487-526.
12. Gehring WJ, Qian YQ, Billeter M, Furukubo-Tokunaga K, Schier AF, Resendez-Perez D,et al. Homeodomain-DNA recognition. Cell 1994;78:211-23.
13. Verrijzer CP, Van der Vliet PC. POU domain transcription factors. Biochim Biophys Acta 1993;1173:1-21.
14. Ryan AK, Rosenfeld MG. POU domain family values: flexibility, partnerships, and developmental codes. Genes Dev 1997;11:1207-25.
15. Wang YP, Song GH, Chen J, Xiao C, Li C, Zhong L, et al. Elevated OCT1 participates in colon tumorigenesis and independently predicts poor prognoses of colorectal cancer patients. Tumour Biol 2016;37:3247-55.
16. Shakya A, Cooksey R, Cox JE, Wang V, McClain DA, Tantin D. OCT1 loss of function induces a coordinate metabolic shift that opposes tumorigenicity. Nat Cell Biol 2009;11:320-7.
17. Boulon S, Dantonel JC, Binet V, Vié A, Blanchard JM, Hipskind RA, Philips A. Oct-1 potentiates CREB-driven cyclin D1 promoter activation via a phospho-CREB- and CREB binding protein-independent mechanism. Mol Cell Biol 2002;22:7769-79.
18. Jin T, Li H. Pou homeodomain protein OCT1 is implicated in the expression of the caudal-related homeobox gene Cdx-2. J Biol Chem 2001;276:14752-8.
19. Bertolino E, Reimund B, Wildt-Perinic D, Clerc RG. A novel homeobox protein which recognizes a TGT core and functionally interferes with a retinoid-responsive motif. J Biol Chem 1995;270:31178-88.
20. Blanco-Arias P,Sargent CA, Affara NA. The human-specific Yp11.2/Xq21.3 homology block encodes a potentially functional testis-specific TGIF-like retroposon. Mamm Genome 2002;13:463-8.
21. Melhuish TA, Gallo CM, Wotton D. TGIF2 interacts with histone deacetylase 1 and represses transcription. J Biol Chem 2001;276:32109-14.
22. Powers SE, Taniguchi K, Yen W, Melhuish TA, Shen J, Walsh CA, et al. Tgif1 and Tgif2 regulate Nodal signaling and are required for gastrulation. Development 2010;137:249-59.
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24. Yu YY, Pan YS, Zhu ZG. Homeobox genes and their functions on development and neoplasm in gastrointestinal tract. Eur J Surg Oncol 2007;33:129-32.
25. Cillo C, Cantile M, Faiella A, Boncinelli E. Homeobox genes in normal and malignant cells. J Cell Physiol 2001;188:161-9.
26. Chen H, Sukumar S. Role of homeobox genes in normal mammary gland development and breast tumorigenesis. J Mammary Gland Biol Neoplasia 200;8:159-75.
27. Reymann S, Borlak J. Transcription profiling of lung adenocarcinomas of c-myc-transgenic mice: identification of the c-myc regulatory gene network. BMC Syst Biol 2008;2:46.
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29. Kim JW, Dang CV. Cancer's molecular sweet tooth and the Warburg effect. Cancer Res 2006;66:8927-30.
30. Almeida R, Almeida J, Shoshkes M, Mendes N, Mesquita P, Silva E,et al. OCT-1 is over-expressed in intestinal metaplasia and intestinal gastric carcinomas and binds to, but does not transactivate, CDX2 in gastric cells. J Pathol 2005;207:396-401.
31. Arber N, Hibshoosh H, Moss SF, Sutter T, Zhang Y, Begg M,et al. Increased expression of cyclin D1 is an early event in multistage colorectal carcinogenesis. Gastroenterology 1996;110:669-74.
32. Bartkova J, Lukas J, Strauss M, Bartek J. The PRAD-1/cyclin D1 oncogene product accumulates aberrantly in a subset of colorectal carcinomas. Int J Cancer 1994;58:568-73.
33. Fan W, Jin S, Tong T, Zhao H, Fan F, Antinore MJ, Rajasekaran B,et al. BRCA1 regulates GADD45 through
its interactions with the OCT-1 and CAAT motifs. J Biol Chem 2002;277:8061-7.
34. Jin S, Fan F, Fan W, Zhao H, Tong T, Blanck P, et al. Transcription factors Oct-1 and NF-YA regulate the p53independent induction of the GADD45 following DNA damage. Oncogene 2001;20:2683-90.
35. Wysocka J, Herr W. The herpes simplex virus VP16-induced complex: the makings of a regulatory switch. Trends Biochem Sci 2003;28:294-304.
36. Tantin D, Schild-Poulter C, Wang V, Haché RJ, Sharp PA. The octamer binding transcription factor Oct-1 is a stress sensor. Cancer Res 2005;65:10750-8.
37. Sebastiano V, Dalvai M, Gentile L, Schubart K, Sutter J, Wu GM, et al. OCT1 regulates trophoblast development during early mouse embryogenesis. Development 2010;137:3551-60.
38. Aarabi M, Ousati-Ashtiani Z, Nazarian A, Modarressi MH, Heidari M. Association of TGIFLX/Y mRNA expression with azoospermia in infertile men. Mol Reprod Dev 2008;75:1761-6.
39. Ousati Ashtiani Z, Ayati M, Modarresi MH, Raoofian R, Sabah Goulian B, Greene WK,et al. Association of TGIFLX/Y mRNA expression with prostate cancer. Med Oncol 2009;26:73-7.
2. Tonini G, Imperatori M, Vincenzi B, Frezza AM, Santini D. Rechallenge therapy and treatment holiday: different strategies in management of metastatic colorectal cancer. J Exp Clin Cancer Res 2013;32:92.
3. Xi RC, Biao WS, Gang ZZ. Significant elevation of survivin and livin expression in human colorectal cancer: inverse correlation between expression and overall survival. Onkologie 2011;34:428-32.
4. Li XD, Miao SY, Wang GL, Yang L, Shu YQ, Yin YM. Amphiregulin and epiregulin expression in colorectal carcinoma and the correlation with clinicopathological characteristics. Onkologie 2010;33:353-8.
5. Wang F, Zhang P, Ma Y, Yang J, Moyer MP, Shi C,et al. NIRF is frequently upregulated in colorectal cancer and its oncogenicity can be suppressed by let-7a microRNA. Cancer Lett 2012;314:223-31.
6. Liang QL, Li ZY, Chen GQ, Lai ZN, Wang BR, Huang J. Prognostic value of serum soluble Fas in patients with locally advanced unresectable rectal cancer receiving concurrent chemoradiotherapy. J Zhejiang Univ Sci B 2010;11:912-7.
7. Liang QL, Wang BR, Li GH. DcR3 and survivin are highly expressed in colorectal carcinoma and closely correlated to its clinicopathologic parameters. J Zhejiang Univ Sci B 2009;10:675-82.
8. Mark M, Rijli FM, Chambon P. Homeobox genes in embryogenesis and pathogenesis. Pediatr Res 1997;42:421-9.
9. Gehring WJ, Hiromi Y. Homeotic genes and the homeobox. Annu Rev Genet 1986;20:147-73.
10. Holland PW, Booth HA, Bruford EA. Classification and nomenclature of all human homeobox genes. BMC Biol 2007;5:47.
11. Gehring, W.J., M. Affolter, and T. Burglin, Homeodomain proteins. Annu Rev Biochem 1994;63:487-526.
12. Gehring WJ, Qian YQ, Billeter M, Furukubo-Tokunaga K, Schier AF, Resendez-Perez D,et al. Homeodomain-DNA recognition. Cell 1994;78:211-23.
13. Verrijzer CP, Van der Vliet PC. POU domain transcription factors. Biochim Biophys Acta 1993;1173:1-21.
14. Ryan AK, Rosenfeld MG. POU domain family values: flexibility, partnerships, and developmental codes. Genes Dev 1997;11:1207-25.
15. Wang YP, Song GH, Chen J, Xiao C, Li C, Zhong L, et al. Elevated OCT1 participates in colon tumorigenesis and independently predicts poor prognoses of colorectal cancer patients. Tumour Biol 2016;37:3247-55.
16. Shakya A, Cooksey R, Cox JE, Wang V, McClain DA, Tantin D. OCT1 loss of function induces a coordinate metabolic shift that opposes tumorigenicity. Nat Cell Biol 2009;11:320-7.
17. Boulon S, Dantonel JC, Binet V, Vié A, Blanchard JM, Hipskind RA, Philips A. Oct-1 potentiates CREB-driven cyclin D1 promoter activation via a phospho-CREB- and CREB binding protein-independent mechanism. Mol Cell Biol 2002;22:7769-79.
18. Jin T, Li H. Pou homeodomain protein OCT1 is implicated in the expression of the caudal-related homeobox gene Cdx-2. J Biol Chem 2001;276:14752-8.
19. Bertolino E, Reimund B, Wildt-Perinic D, Clerc RG. A novel homeobox protein which recognizes a TGT core and functionally interferes with a retinoid-responsive motif. J Biol Chem 1995;270:31178-88.
20. Blanco-Arias P,Sargent CA, Affara NA. The human-specific Yp11.2/Xq21.3 homology block encodes a potentially functional testis-specific TGIF-like retroposon. Mamm Genome 2002;13:463-8.
21. Melhuish TA, Gallo CM, Wotton D. TGIF2 interacts with histone deacetylase 1 and represses transcription. J Biol Chem 2001;276:32109-14.
22. Powers SE, Taniguchi K, Yen W, Melhuish TA, Shen J, Walsh CA, et al. Tgif1 and Tgif2 regulate Nodal signaling and are required for gastrulation. Development 2010;137:249-59.
23. Lai YL, Li H, Chiang HS, Hsieh-Li HM. Expression of a novel TGIF subclass homeobox gene, Tex1, in the spermatids of mouse testis during spermatogenesis. Mech Dev 2002;113:185-7.
24. Yu YY, Pan YS, Zhu ZG. Homeobox genes and their functions on development and neoplasm in gastrointestinal tract. Eur J Surg Oncol 2007;33:129-32.
25. Cillo C, Cantile M, Faiella A, Boncinelli E. Homeobox genes in normal and malignant cells. J Cell Physiol 2001;188:161-9.
26. Chen H, Sukumar S. Role of homeobox genes in normal mammary gland development and breast tumorigenesis. J Mammary Gland Biol Neoplasia 200;8:159-75.
27. Reymann S, Borlak J. Transcription profiling of lung adenocarcinomas of c-myc-transgenic mice: identification of the c-myc regulatory gene network. BMC Syst Biol 2008;2:46.
28. Zheng L, Roeder RG, Luo Y. S phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component. Cell 2003;114:255-66.
29. Kim JW, Dang CV. Cancer's molecular sweet tooth and the Warburg effect. Cancer Res 2006;66:8927-30.
30. Almeida R, Almeida J, Shoshkes M, Mendes N, Mesquita P, Silva E,et al. OCT-1 is over-expressed in intestinal metaplasia and intestinal gastric carcinomas and binds to, but does not transactivate, CDX2 in gastric cells. J Pathol 2005;207:396-401.
31. Arber N, Hibshoosh H, Moss SF, Sutter T, Zhang Y, Begg M,et al. Increased expression of cyclin D1 is an early event in multistage colorectal carcinogenesis. Gastroenterology 1996;110:669-74.
32. Bartkova J, Lukas J, Strauss M, Bartek J. The PRAD-1/cyclin D1 oncogene product accumulates aberrantly in a subset of colorectal carcinomas. Int J Cancer 1994;58:568-73.
33. Fan W, Jin S, Tong T, Zhao H, Fan F, Antinore MJ, Rajasekaran B,et al. BRCA1 regulates GADD45 through
its interactions with the OCT-1 and CAAT motifs. J Biol Chem 2002;277:8061-7.
34. Jin S, Fan F, Fan W, Zhao H, Tong T, Blanck P, et al. Transcription factors Oct-1 and NF-YA regulate the p53independent induction of the GADD45 following DNA damage. Oncogene 2001;20:2683-90.
35. Wysocka J, Herr W. The herpes simplex virus VP16-induced complex: the makings of a regulatory switch. Trends Biochem Sci 2003;28:294-304.
36. Tantin D, Schild-Poulter C, Wang V, Haché RJ, Sharp PA. The octamer binding transcription factor Oct-1 is a stress sensor. Cancer Res 2005;65:10750-8.
37. Sebastiano V, Dalvai M, Gentile L, Schubart K, Sutter J, Wu GM, et al. OCT1 regulates trophoblast development during early mouse embryogenesis. Development 2010;137:3551-60.
38. Aarabi M, Ousati-Ashtiani Z, Nazarian A, Modarressi MH, Heidari M. Association of TGIFLX/Y mRNA expression with azoospermia in infertile men. Mol Reprod Dev 2008;75:1761-6.
39. Ousati Ashtiani Z, Ayati M, Modarresi MH, Raoofian R, Sabah Goulian B, Greene WK,et al. Association of TGIFLX/Y mRNA expression with prostate cancer. Med Oncol 2009;26:73-7.
| Files | ||
| Issue | Vol 56, No 10 (2018) | |
| Section | Articles | |
| Keywords | ||
| Colorectal cancer TGIFLX OCT1 Real time RT-PCR | ||
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How to Cite
1.
Sadeghipour N, Lotfiyani F, Raoofian R, Noori-Daloii M-R, Azimi C, Heidari M. Investigation of mRNA Expression Levels of TGIFLX and OCT1 Homeobox Genes in Colorectal Cancer. Acta Med Iran. 2019;56(10):653-659.
