Angiogenesis and Mast Cells Density in Oral and Esophageal Squamous Cell Carcinomas
-
Sadra Masali
,
Rosa Miri
,
Mojgan Alaeddini
,
Shahroo Etemad-Moghadam
,
Donia Sadri
,
Fatemeh Shahsavari
Abstract
Despite the similarities between oral and esophageal squamous cell carcinomas (SCCs), the survival rate of this cancer is relatively low in the esophagus compared to the oral cavity. To our knowledge, mast cells and angiogenesis have not been simultaneously compared between oral and esophageal SCCs. However, they have been separately evaluated in each of these locations with conflicting results. Therefore, the aim of this study was to assess and compare mast cell count and microvessel density between SCCs of the esophagus and oral cavity. A total of 46 oral and esophageal SCCs (23 of each) were stained immunohistochemically and histochemically with CD31 and methylene blue, respectively. Statistical analysis was performed using t-test, one-way ANOVA, and Pearson correlation analysis. Microvessel density was significantly higher in oral compared to esophageal tumors (P=0.02). Conversely, esophageal SCCs showed significantly higher mast cell counts than that of oral neoplasms (P=0.04). Pearson correlation analysis showed no association between these two factors in either oral SCC (P=0.51) or esophageal SCC (P=0.34). A significant difference between mean mast cell count and microvessel density in oral and esophageal SCC may be related to inherent differences in the tissues of origin and might, to some extent, be responsible for the different biological behaviors of these cancers.
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13) Broders AC. The microscopic grading of cancer. Surg Clin North Am 1941;21:947-62.
14) Weidner N, Semple JP, Welch WR, Folkman J. Tumor angiogenesis and metastasis--correlation in invasive breast carcinoma. N Engl J Med. 1991 Jan 3;324(1):1-8.
15) Tang XH, Knudsen B, Bemis D, Tickoo S, Gudas LJ. Oral cavity and esophageal carcinogenesis modeled in carcinogen-treated mice. Clin Cancer Res. 2004 Jan 1;10(1 Pt 1):301-13.
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18) Rigoni A, Colombo MP, Pucillo C. The Role of Mast Cells in Molding the Tumor Microenvironment. Cancer Microenviron. 2015 Dec;8(3):167-76.
19) Shahsavari F, Farhadi S, Sadri D, Sedehi M. Evaluation of Microvascularity by CD34 Expression in Esophagus and Oral Squamous Cell Carcinoma. J Contemp Dent Pract. 2015 Jun 1;16(6):458-62.
20) Pusztaszeri MP, Seelentag W, Bosman FT. Immunohistochemical expression of endothelial markers CD31, CD34, von Willebrand factor, and Fli-1 in normal human tissues. J Histochem Cytochem. 2006 Apr;54(4):385-95.
21) Yokota J. Tumor progression and metastasis. Carcinogenesis. 2000 Mar;21(3):497-503.
22) Jung I, Gurzu S, Raica M, Cîmpean AM, Szentirmay Z. The differences between the endothelial area marked with CD31 and CD105 in colorectal carcinomas by computer-assisted morphometrical analysis. Rom J Morphol Embryol. 2009;50(2):239-43.
23) Pujari RK, Vanaki SS, Puranik RS, Desai RS, Motupalli N, Halawar S. Histomorphometric analysis of vascularity in normal buccal mucosa, leukoplakia, and squamous cell carcinoma of buccal mucosa. J Oral Maxillofac Pathol. 2013 Sep;17(3):334-9
24) Parizi AC, Barbosa RL, Parizi JL, Nai GA. A comparison between the concentration of mast cells in squamous cell carcinomas of the skin and oral cavity. An Bras Dermatol. 2010 Nov-Dec;85(6):811-8.
25) Yu Y, Ding X, Wang O, Xie L, Hu W, Chen K. Alterations of Mast Cells in the Esophageal Mucosa of the Patients With Non-Erosive Reflux Disease. Gastroenterol Res. 2011;4:70–75.
26) Ingaleshwar PS, Pandit S, Desai D, Redder CP, Shetty AS, Mithun KM. Immunohistochemical analysis of angiogenesis by CD34 and mast cells by toluidine blue in different grades of oral squamous cell carcinoma. J Oral Maxillofac Pathol. 2016 Sep-Dec;20(3):467-473.
27) Tahir A, Nagi AH, Ullah E, Janjua OS. The role of mast cells and angiogenesis in well-differentiated oral squamous cell carcinoma. J Cancer Res Ther. 2013 Jul-Sep;9(3):387-91.
28) Jahanshahi G, Sabaghian M. Comparative immunohistochemical analysis of angiogenesis and mast cell density in oral normal mucosa and squamous cell carcinoma. Dent Res J (Isfahan). 2012 Jan;9(1):8-12.
2) Su XD, Zhang DK, Zhang X, Lin P, Long H, Rong TH. Prognostic factors in patients with recurrence after complete resection of esophageal squamous cell carcinoma. J Thorac Dis. 2014 Jul;6(7):949-57.
3) Gavish A, Krayzler E, Nagler R. Tumor Growth and Cell Proliferation Rate in Human Oral Cancer. Arch Med Res. 2016 May;47(4):271-4.
4) Szymańska K, Hung RJ, Wünsch-Filho V, Eluf-Neto J, Curado MP, Koifman S, Matos E, Menezes A, Fernandez L, Daudt AW, Boffetta P, Brennan P. Alcohol and tobacco, and the risk of cancers of the upper aerodigestive tract in Latin America: a case-control study. Cancer Causes Control. 2011 Jul;22(7):1037-46.
5) Etemad-Moghadam S, Khalili M, Tirgary F, Alaeddini M. Evaluation of myofibroblasts in oral epithelial dysplasia and squamous cell carcinoma. J Oral Pathol Med. 2009 Sep;38(8):639-43.
6) Eckert AW, Wickenhauser C, Salins PC, Kappler M, Bukur J, Seliger B. Clinical relevance of the tumor microenvironment and immune escape of oral squamous cell carcinoma. J Transl Med. 2016 Apr 5;14:85.
7) Agrawal U, Rai H, Jain AK. Morphological and ultrastructural characteristics of extracellular matrix changes in oral squamous cell carcinoma. Indian J Dent Res. 2011 Jan-Feb;22(1):16-21.
8) Koontongkaew S. The tumor microenvironment contribution to development, growth, invasion and metastasis of head and neck squamous cell carcinomas. J Cancer. 2013;4(1):66-83.
9) Li H, Fan X, Houghton J. Tumor microenvironment: the role of the tumor stroma in cancer. J Cell Biochem. 2007 Jul 1;101(4):805-15.
10) Kalra M, Rao N, Nanda K, Rehman F, Girish KL, Tippu S, Arora A. The role of mast cells on angiogenesis in oral squamous cell carcinoma. Med Oral Patol Oral Cir Bucal. 2012 Mar 1;17(2):e190-6.
11) Elpek GO, Gelen T, Aksoy NH, Erdoğan A, Dertsiz L, Demircan A, Keleş N. The prognostic relevance of angiogenesis and mast cells in squamous cell carcinoma of the oesophagus. J Clin Pathol. 2001 Dec;54(12):940-4.
12) Iamaroon A, Pongsiriwet S, Jittidecharaks S, Pattanaporn K, Prapayasatok S, Wanachantararak S. Increase of mast cells and tumor angiogenesis in oral squamous cell carcinoma. J Oral Pathol Med. 2003 Apr;32(4):195-9.
13) Broders AC. The microscopic grading of cancer. Surg Clin North Am 1941;21:947-62.
14) Weidner N, Semple JP, Welch WR, Folkman J. Tumor angiogenesis and metastasis--correlation in invasive breast carcinoma. N Engl J Med. 1991 Jan 3;324(1):1-8.
15) Tang XH, Knudsen B, Bemis D, Tickoo S, Gudas LJ. Oral cavity and esophageal carcinogenesis modeled in carcinogen-treated mice. Clin Cancer Res. 2004 Jan 1;10(1 Pt 1):301-13.
16) Wenig BM. Squamous cell carcinoma of the upper aerodigestive tract: precursors and problematic variants. Mod Pathol. 2002 Mar;15(3):229-54.
17) Quail DF, Joyce JA. Microenvironmental regulation of tumor progression and metastasis. Nat Med. 2013 Nov;19(11):1423-37.
18) Rigoni A, Colombo MP, Pucillo C. The Role of Mast Cells in Molding the Tumor Microenvironment. Cancer Microenviron. 2015 Dec;8(3):167-76.
19) Shahsavari F, Farhadi S, Sadri D, Sedehi M. Evaluation of Microvascularity by CD34 Expression in Esophagus and Oral Squamous Cell Carcinoma. J Contemp Dent Pract. 2015 Jun 1;16(6):458-62.
20) Pusztaszeri MP, Seelentag W, Bosman FT. Immunohistochemical expression of endothelial markers CD31, CD34, von Willebrand factor, and Fli-1 in normal human tissues. J Histochem Cytochem. 2006 Apr;54(4):385-95.
21) Yokota J. Tumor progression and metastasis. Carcinogenesis. 2000 Mar;21(3):497-503.
22) Jung I, Gurzu S, Raica M, Cîmpean AM, Szentirmay Z. The differences between the endothelial area marked with CD31 and CD105 in colorectal carcinomas by computer-assisted morphometrical analysis. Rom J Morphol Embryol. 2009;50(2):239-43.
23) Pujari RK, Vanaki SS, Puranik RS, Desai RS, Motupalli N, Halawar S. Histomorphometric analysis of vascularity in normal buccal mucosa, leukoplakia, and squamous cell carcinoma of buccal mucosa. J Oral Maxillofac Pathol. 2013 Sep;17(3):334-9
24) Parizi AC, Barbosa RL, Parizi JL, Nai GA. A comparison between the concentration of mast cells in squamous cell carcinomas of the skin and oral cavity. An Bras Dermatol. 2010 Nov-Dec;85(6):811-8.
25) Yu Y, Ding X, Wang O, Xie L, Hu W, Chen K. Alterations of Mast Cells in the Esophageal Mucosa of the Patients With Non-Erosive Reflux Disease. Gastroenterol Res. 2011;4:70–75.
26) Ingaleshwar PS, Pandit S, Desai D, Redder CP, Shetty AS, Mithun KM. Immunohistochemical analysis of angiogenesis by CD34 and mast cells by toluidine blue in different grades of oral squamous cell carcinoma. J Oral Maxillofac Pathol. 2016 Sep-Dec;20(3):467-473.
27) Tahir A, Nagi AH, Ullah E, Janjua OS. The role of mast cells and angiogenesis in well-differentiated oral squamous cell carcinoma. J Cancer Res Ther. 2013 Jul-Sep;9(3):387-91.
28) Jahanshahi G, Sabaghian M. Comparative immunohistochemical analysis of angiogenesis and mast cell density in oral normal mucosa and squamous cell carcinoma. Dent Res J (Isfahan). 2012 Jan;9(1):8-12.
| Files | ||
| Issue | Vol 57, No 10 (2019) | |
| Section | Articles | |
| DOI | https://doi.org/10.18502/acta.v57i10.3230 | |
| Keywords | ||
| Oral squamous cell carcinoma Esophageal squamous cell carcinoma Microvessel density CD31 Mast cells | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Masali S, Miri R, Alaeddini M, Etemad-Moghadam S, Sadri D, Shahsavari F. Angiogenesis and Mast Cells Density in Oral and Esophageal Squamous Cell Carcinomas. Acta Med Iran. 2020;57(10):575-580.
