Prevalence of Helicobacter pylori vacA, cagA, cagE1, cagE2, dupA and oipA Genotypes in Patients With Gastrointestinal Diseases

  • Ali badamchi Mail TehranUniversity of Medical Sciences, Tehran, Iran
Helicobacter pylori, Vacuolating cytotoxin gene A (vacA), Cytotoxin-associated gene A (cagA), Cytotoxin-associated gene E1 (cagE1), Cytotoxin-associated gene E2 (cagE2), Duodenal ulcer promoting gene A (dupA)



Helicobacter pylori (H. pylori) is a bacterium resides in the human stomach which is associated with gastroduodenal diseases. We investigate the prevalence of cagA, vacA, oipA, cagE1, cagE2 and dupA genotypes in H. pylori isolated from patients with Gastric ulcer, duodenal ulcer, and Gastric Cancer.


 Collected 74 samples from the Gastroenterology Unit of the Rasool Akram Hospital were included in this study. Gastric disorders were identified by endoscopy .gastric cancer was further confirmed by histopathology. H. pylori were detected by the urease test. Subsequently, DNA was extracted from gastric tissue of the subjects with the CLO-test yielded positive results.


In general, 74 patients with a mean age of 53.45 years (Range 22 to 86 years old), including 45 men and 29 women, were studied.  Among 74 H. pylori-positive patients, 70 (94.5%) patients were positive for the cagA gene. About 95.8% (23/24) of the patients with gastric carcinoma were dupA positive and VacA gene (91.8%). The oipA genotype was detected in 71 (96%) of H.pylori positive samples. This gene was more common in patients with gastritis rather than cancer group. Also, 97.2% of 74 H. pylori isolates were cagE2-positive. In 25 patients with PUD, the occurrence percent of cagA+/VacA+, cagA+/Vac- , cagA- /VacA+ and cagA- /VaxA- genotypes were found 80%, 12%, 4.2% and 4.2 respectively.


 The results of the present study suggest that a high prevalence of virulent factors could contribute to the risk of developing gastroduodenal diseases.


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How to Cite
badamchi A. Prevalence of Helicobacter pylori vacA, cagA, cagE1, cagE2, dupA and oipA Genotypes in Patients With Gastrointestinal Diseases. Acta Med Iran. 58(7):310-317.