Resistance to Antimicrobial Agents, Chemical Disinfectants and Distribution of Effectors Proteins of Type Three Secretion System in Pseudomonas aeruginosa Isolated From Burn and Hospital Environments in South East of Iran

  • Mehdi Rahmati Microbiology Department, Kerman University of Medical Sciences, Kerman, Iran
  • Shahla Mansouri Mail Microbiology Department, Kerman University of Medical Sciences, Kerman, Iran
  • Hosein Sharifi Microbiology Department, Kerman University of Medical Sciences, Kerman, Iran
Keywords:
P. aeruginosa, Burn, Antibacterial resistance, Chemical disinfectant, Type three secretion system

Abstract

Pseudomonas aeruginosa (P. aeruginosa) is a common bacteria associated with burn infections and resistance to a wide range of disinfectants and antimicrobial agents which is able to produce different virulence factors. In this study, the susceptibility of P. aeruginosa isolates from burn (burn=57) and hospital environment (HE=19) to antimicrobial agents and chemical disinfectants was determined by disc and well diffusion agar method, respectively. The results showed 100% sensitivity to polymyxin B, while sensitivity to other agents were low and ranged from 40.8% for imipenem and amikacin to 6.6% for ceftizoxime. Among the disinfectant used the mean diameter of inhibition zones (DIZ) was higher for deconex while nitrofurazone had the lowest DIZ. In most cases the HE isolates were significantly more susceptible to disinfectants and antimicrobial agents compared to burn isolates (P≤0.01). The genes for the exoenzyme T, Y, U and S were detected in 100%, 89.8%, 43.4% and 48.7% of the isolates respectively. Prevalence of exo U and exoY was significantly higher in the burn isolates compared to HE isolates (P=0.001). The results of this study indicate significantly higher level of resistance against the majority of the antimicrobial agents in the burn isolates compared to HE isolates which was significantly higher than the environmental isolates. The prevalence of T3SS effectors proteins and their pattern were also different in the burn and the HE isolates, indicating a divergence in pathogenicity of the burn isolates from those of the environmental isolates.

References

1. Bennett JV. Nosocomial infections due to Pseudomonas. J Infect Dis. 1974;130(1):4-7.
2. Smith EE, Buckley DG, Wu Z, Saenphimmachak C, Hoffman LR, D’Argenio DA, et al. Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients. Proc Natl Acad Sci. 2006;103(22):8487-92.
3. Pirnay J-P, De Vos D, Cochez C, Bilocq F, Pirson J, Struelens M, et al. Molecular epidemiology of Pseudomonas aeruginosa colonization in a burn unit: persistence of a multidrug-resistant clone and a silver sulfadiazine-resistant clone. J Clin Microbiol. 2003;41(3):1192-202.
4. Biswal I, Arora BS, Dimple Kasana N. Incidence of multidrug resistant Pseudomonas aeruginosa isolated from burn patients and environment of teaching institution. J Clin Diagn Res. 2014;8(5):26-9.
5. Engel J, Balachandran P. Role of Pseudomonas aeruginosa type III effectors in disease. Curr Opin Microbiol. 2009;12(1):61-6.
6. Lee VT, Smith RS, Tümmler B, Lory S. Activities of Pseudomonas aeruginosa effectors secreted by the Type III secretion system in vitro and during infection. Infect Immun. 2005;73(3):1695-705.
7. Wong-Beringer A, Wiener-Kronish J, Lynch S, Flanagan J. Comparison of type III secretion system virulence among fluoroquinolone-susceptible and-resistant clinical isolates of Pseudomonas aeruginosa. Clin Microbiol Infect. 2008;14(4):330-6.
8. Lyczak JB, Cannon CL, Pier GB. Establishment of Pseudomonas aeruginosa infection: lessons from a versatile opportunist. Microbes Infect. 2000;2(9):1051-60.
9. Pirnay J-P, Bilocq F, Pot B, Cornelis P, Zizi M, Van Eldere J, et al. Pseudomonas aeruginosa population structure revisited. PLoS One. 2009;4(11):e7740.
10. Finnan S, Morrissey JP, O'gara F, Boyd EF. Genome diversity of Pseudomonas aeruginosa isolates from cystic fibrosis patients and the hospital environment. J Clin Microbiol. 2004;42(12):5783-92.
11. Pitout JD, Gregson DB, Poirel L, McClure J-A, Le P, Church DL. Detection of Pseudomonas aeruginosa producing metallo-β-lactamases in a large centralized laboratory. J Clin Microbiol. 2005;43(7):3129-35.
12. Strateva T, Markova B, Ivanova D, Mitov I. Distribution of the type III effector proteins-encoding genes among nosocomial Pseudomonas aeruginosa isolates from Bulgaria. Ann Microbiol. 2010;60(3):503-9.
13. Hurst V, Sutter VL. Survival of Pseudomonas aeruginosa in the hospital environment. J Infect Dis. 1966;116(2):151-4.
14. Choy MH, Stapleton F, Willcox MD, Zhu H. Comparison of virulence factors in Pseudomonas aeruginosa strains isolated from contact lens-and non-contact lens-related keratitis. J Med Microbiol. 2008;57(12):1539-46.
15. Rafla K, Tredget EE. Infection control in the burn unit. Burns. 2011;37(1):5-15.
16. Estahbanati HK, Kashani PP, Ghanaatpisheh F. Frequency of Pseudomonas aeruginosa serotypes in burn wound infections and their resistance to antibiotics. Burns. 2002;28(4):340-8.
17. Nikbin V, Aslani MM, Sharafi Z, Hashemipour M, Shahcheraghi F, Ebrahimipour G. Molecular identification and detection of virulence genes among Pseudomonas aeruginosa isolated from different infectious origins. Iran J Microbiol. 2012;4(3):118-23.
18. Janjua HA, Segata N, Bernabo P, Tamburini S, Ellen A, Jousson O. Clinical populations of Pseudomonas aeruginosa isolated from acute infections show a wide virulence range partially correlated with population structure and virulence gene expression. Microbiology. 2012;158(8):2089-98.
19. Badrian H, Ghasemi E, Khalighinejad N, Hosseini N. The effect of three different disinfection materials on alginate impression by spray method. ISRN Dent. 2012;2012(695151):1-5.
20. Japoni A, Hayati M, Alborzi A, Farshad S, Abbasian S. In vitro susceptibility of Pseudomonas aeruginosa isolated from a burn center to silver sulfadiazine and silver nitrate in Shiraz, South of Iran. Iran J Med Sci. 2005;30(2):63-7.
21. Romão CMCPA, Faria YNd, Pereira LR, Asensi MD. Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing. Mem Inst Oswaldo Cruz. 2005;100(5):541-8.
22. Barbier M, Damron FH, Bielecki P, Suárez-Diez M, Puchałka J, Albertí S, et al. From the environment to the host: re-wiring of the transcriptome of Pseudomonas aeruginosa from 22 C to 37 C. PLoS One. 2014;9(2):e89941.
23. Deptuła A, Gospodarek E. Reduced expression of virulence factors in multidrug-resistant Pseudomonas aeruginosa strains. Arch Microbiol. 2010;192(1):79-84.
24. Di Martino P, Sirot D, Joly B, Rich C, Darfeuille-Michaud A. Relationship between adhesion to intestinal Caco-2 cells and multidrug resistance in Klebsiella pneumoniae clinical isolates. J Clin Microbiol. 1997;35(6):1499-503.
25. Feltman H, Schulert G, Khan S, Jain M, Peterson L, Hauser AR. Prevalence of type III secretion genes in clinical and environmental isolates of Pseudomonas aeruginosa. Microbiology. 2001;147(10):2659-69.
26. Mitov I, Strateva T, Markova B. Prevalence of virulence genes among bulgarian nosocomial and cystic fibrosis isolates of Pseudomonas aeruginosa. Braz J Microbiol. 2010;41(3):588-95.
Published
2020-10-18
How to Cite
1.
Rahmati M, Mansouri S, Sharifi H. Resistance to Antimicrobial Agents, Chemical Disinfectants and Distribution of Effectors Proteins of Type Three Secretion System in Pseudomonas aeruginosa Isolated From Burn and Hospital Environments in South East of Iran. Acta Med Iran. 58(7):345-351.
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Articles