The association of extravasated platelet aggregation with clinical futures in patients with colorectal cancer and its correlation with EMT markers

  • Naghmeh Ahmadiankia ORCID Mail School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran. AND Cancer Prevention Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.
  • Maryam Yarmohammadi ORCID Imam Hossein Center for Education, Research and Treatment, Shahroud University of Medical Sciences, Shahroud, Iran.
  • Azam Hadi ORCID Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
  • Behnaz Jafari ORCID School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
  • Mozhgan Fazli ORCID School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
  • Zahra Shahmohammadi ORCID Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
Keywords:
Clinical futures, Colorectal cancer, Epithelial-mesenchymal transition, Platelets

Abstract

The theory of platelet role in cancer progression was recently introduced. We investigated the association of extravasated platelets in colorectal cancer with clinicopathological features, and also the expression of epithelial-mesenchymal transition (EMT) markers. We retrospectively analyzed data from 33 patients with colorectal cancer who underwent surgery between 2013-2016. In formalin-fixed paraffin-embedded tissues, we evaluated the expression of a platelet-specific marker of CD42b and EMT markers using immunohistochemistry. The associations among the expression of the platelet‑specific marker in specimens, EMT, and clinicopathological futures were analyzed. The presence of platelets was observed in 15 out of 33 primary colorectal tumors (45%). According to multivariate analysis, CD42b expression was not correlated with clinical characteristics. Platelet-positive tumor cells did not show EMT marker expression. These data suggest that extravasated platelets may not have a central role in determining patient characteristics and clinical futures.

References

1.Siegel RL, Miller KD, Jemal A. Cancer statistics, 2019. CA Cancer J Clin 2019;69:7-34.
2.Siegel RL, Miller KD, Jemal A. Cancer statistics, 2018. CA Cancer J Clin 2018;68:7-30.
3.Mishra J, Drummond J, Quazi SH, Karanki SS, Shaw JJ, Chen B, et al. Prospective of colon cancer treatments and scope for combinatorial approach to enhanced cancer cell apoptosis. Crit Rev Oncol Hematol 2013;86:232- 0.
4.Augestad KM, Bakaki PM, Rose J, Crawshaw BP, Lindsetmo RO, Dorum LM, et al. Metastatic spread pattern after curative colorectal cancer surgery. A retrospective, longitudinal analysis. Cancer Epidemiol 2015;39:734-44.
5.Ungefroren H, Sebens S, Seidl D, Lehnert H, Hass R. Interaction of tumor cells with the microenvironment. Cell Commun Signal2011;9:18.
6.Naderi-Meshkin H, Ahmadiankia N. Cancer metastasis versus stem cell homing: Role of platelets. J Cell Physiol2018;233:9167-78.
7.Golebiewska EM, Poole AW. Platelet secretion: From haemostasis to wound healing and beyond. Blood Rev2015;29:153-62.
8.Cao H, Xu E, Liu H, Wan L, Lai M. Epithelial-mesenchymal transition in colorectal cancer metastasis: A system review. Pathol Res Pract2015;211:557-69.
9.Vu T, Datta PK. Regulation of EMT in Colorectal Cancer:A Culprit in Metastasis. Cancers 2017;9:171.
10.Elzagheid A, Buhmeida A, Laato M, El-Faitori O, Syrjanen K, Collan Y, et al. Loss of E-cadherin expression predicts disease recurrence and shorter survival in colorectal carcinoma. APMIS 2012;120:539-48.
11.Yun JA, Kim SH, Hong HK, Yun SH, Kim HC, Chun HK, et al. Loss of E-Cadherin expression is associated with a poor prognosis in stage III colorectal cancer. Oncology 2014;86:318-28.
12.Niknami Z, Eslamifar A, Emamirazavi A, Ebrahimi A, Shirkoohi R. The association of vimentin and fibronectin gene expression with epithelial-mesenchymal transition and tumor malignancy in colorectal carcinoma. EXCLI j2017;16:1009-17.
13.Mao Y, Xu J, Li Z, Zhang N, Yin H, Liu Z. The role of nuclear beta-catenin accumulation in the Twist2-induced ovariancancer EMT. PloS one 2013;8:e78200.
14.Li S, Luo W. Matrix metalloproteinase 2 contributes to aggressive phenotype, epithelial-mesenchymal transition and poor outcome in nasopharyngeal carcinoma. Onco Targets Ther2019;12:5701-11.
15.GerashchenkoTS, Novikov NM, Krakhmal NV, Zolotaryova SY, Zavyalova MV, Cherdyntseva NV, et al. Markers of Cancer Cell Invasion: Are They Good Enough? J clin med 2019;8:1092.
16.Ishikawa S, Miyashita T, Inokuchi M, Hayashi H, Oyama K, Tajima H, et al. Platelets surrounding primary tumor cells are related to chemoresistance. Oncol rep 2016;36:787-94.
17.Karatzas G, Karayiannakis AJ, Syrigos KN, Chatzigianni E, Papanikolaou S, Riza F, et al. E-cadherin expression correlates with tumor differentiation in colorectal cancer. Hepatogastroenterology1999;46:232-5.
18.Fuyuhiro Y, Yashiro M, Noda S, Kashiwagi S, Matsuoka J, Doi Y, et al. Clinical significance of vimentin-positive gastric cancer cells.Anticancer Res.2010;30:5239-43.
19.Lee KS, Kwak Y, Nam KH, Kim DW, Kang SB, Choe G, et al. Favorable prognosis in colorectal cancer patients with co-expression of c-MYC and ss-catenin. BMC cancer 2016;16:730
.20.Cho YR, Kwon HC, Suh SH, Lee JH, Kim SH, Choi HJ, et al. Expressions of matrix metalloproteinase-7 and -9 and their prognostic significances in rectal cancer. Cancer Res Treat2005;37:354-9.
21.Miyashita T, Tajima H, Makino I, Nakagawara H, Kitagawa H, Fushida S, et al. Metastasis-promoting role of extravasated platelet activation in tumor. J Surg Res2015;193:289-94.
22.Qi C, Li B, Guo S, Wei B, Shao C, Li J, et al. P-Selectin-Mediated Adhesion between Platelets and Tumor Cells Promotes Intestinal Tumorigenesis in Apc(Min/+) Mice. Int J Biol Sci2015;11:679-87.
23.Mikami J, Kurokawa Y, Takahashi T, Miyazaki Y, Yamasaki M, Miyata H, et al. Antitumor effect of antiplatelet agents in gastric cancer cells: an in vivo and in vitro study. Gastric Cancer2016;19:817-26.
Published
2020-08-19
How to Cite
1.
Ahmadiankia N, Yarmohammadi M, Hadi A, Jafari B, Fazli M, Shahmohammadi Z. The association of extravasated platelet aggregation with clinical futures in patients with colorectal cancer and its correlation with EMT markers. Acta Med Iran. 58(6):266-270.
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Articles