Acta Medica Iranica 2018. 56(7):434-440.

Detection and Genotyping of Epstein-Bar Virus Among Paraffin Embedded Tissues of Hodgkin and Non-Hodgkin's Lymphoma Patients in Ahvaz, Iran
Ala Habibian, Manoochehr Makvandi, Alireza Samarbaf-Zadeh, Niloofar Neisi, Rahim Soleimani-Jelodar, Kamyar Makvandi, Shima Izadi

Abstract


Epstein-Barr virus (EBV) is a DNA virus which belongs to the Herpesviridae family, has been infected with 90% of the world’s population. EBV is transmitted through saliva and associated with different diseases such as Infectious Mononucleosis, Nasopharyngeal Carcinoma, Burkitt`s lymphoma, Hodgkin and Non-Hodgkin`s lymphoma. This study was designed to determine the frequency of EBV DNA and genotyping of this virus in histological tissues of Hodgkin and Non-Hodgkin's lymphoma in Ahvaz, Iran. In this study, 12 samples of Hodgkin and 29 samples of Non-Hodgkin`s lymphoma were examined from Ahvaz Imam Khomeini Hospital. After deparaffinization and DNA extraction, Nested-PCR technique was carried out on Epstein-Barr nuclear antigen 1 (EBNA-1) and Epstein-Barr nuclear antigen 3C (EBNA-3C) regions in order to detect EBV genome and genotype in tumoral tissues. Among 12 cases of Hodgkin`s lymphoma, 3 (25%) cases and among 29 cases of Non-Hodgkin's lymphoma, 14 (48%) cases were positive for EBV. All 3 EBV positive cases of Hodgkin`s lymphoma and a number of 8 (57%) cases of Non-Hodgkin`s lymphoma belonged to the adolescence age group which proves an association between age and EBV positive in Hodgkin and Non-Hodgkin`s lymphoma according to Fisher`s exact test (P=0.03). All EBV positive cases in Hodgkin and Non-Hodgkin`s lymphoma were EBV-1. The results of this study revealed that EBV played a possible important role in Hodgkin and Non-Hodgkin`s lymphoma especially in adults. Also, the main genotype of virus in the current study was EBV-1.


Keywords


EBNA-1; EBNA-3C; Epstein-Barr virus; Hodgkin lymphoma; Non-hodgkin lymphoma

Full Text:

PDF

References


Arvin A, Campadelli G, Mocarski E, Moore PRB, Whitley R, Yamanishi K. Human Herpes Virus Arvin A, Campadelli G, Mocarski E, Moore PRB, Whitley R, Yamanishi K, editors. New York: Cambridge University ; 2007.

Brink A, Vervoort M, Middeldorp J, Meijer C, van den Brule A. Nucleic Acid Sequence-Based Amplification, a New Method for Analysis of Spliced and Unspliced Epstein-Barr Virus Latent Transcripts, and Its Comparison with Reverse Transcriptase PCR. J. Clin. Microbiol. 1998; 36(11): 3164-3169.

Fields BN, Knipe DM, Howley PM. Fields Virology. 6th ed. Cohen JI, Griffin DE, Lamb RA, Martin MA, Racaniello VR, Roizman B, editors. Philadelphia: Lippincott Williams & Wilkins; 2013.

Pereira de Lima MA, Pitombeira Ferreira MV, Pessoa Barros MA, Inês de Moura Campos Pardini M, Camargo Ferrasi A, Barem Rabenhorst SH. Epstein-Barr virus-associated gastric carcinoma in Brazil: comparison between in situ hybridization and polymerase chain reaction detection. Braz J Microbiol. 2012 Jan./Mar.; 43(1): 393-404.

Klein G, Klein E, Kashuba E. Interaction of Epstein-Barr virus (EBV) with human B-lymphocytes. Biochemical and Biophysical Research Communications. 2010; 396: 67-73.

Stevens SJ, Vervoort MB, van den Brule A, Meenhorst PL, Meijer C, Middeldorp J. Monitoring of Epstein-Barr Virus DNA Load in Peripheral Blood by Quantitative Competitive PCR. J Clin Microbiol. 1999, 37(9): 2852–7.

Carbone A, Gloghini A, Dotti G. EBV-Associated Lymphoproliferative Disorders: Classification and Treatment. Oncologist. 2008; 13: 577-85.

Ai J, Xie Z, Liu C, Huang Z, Xu J. Analysis of EBNA-1 and LMP-1 variants in diseases associated with EBV infection in Chinese children. Virol J. 2012; 9(13), doi:10.1186/1743-422X-

Hassan R, White LR, Gustavo Stefanoff C, Elgui de Oliveira D, Felisbino F, Esteves Klumb C, et al. Epstein-Barr Virus (EBV) detection and typing by PCR: a contribution to diagnostic screening of EBV-positive Burkitt's lymphoma. Diagn Pathol. 2006; 1(17), doi:10.1186/1746-1596-1-17.

Kim I, Park ER, Park SH, Lin Z, Kim YS. Characteristics of Epstein-Barr Virus Isolated From The Malignant Lymphomas in Korea. J Med Virol. 2002; 67: 59-66.

Ocheni S, Olusina DB, Oyekunle AA, Ibegbulam OG, Kröger N, Bacher U, et al. EBV-Associated Malignancies. The Open Infect Dis J. 2010; 4: 101-112.

Wang WY, Chien YC, Jan JS, Chueh CM, Lin JC. Consistent Sequence Variation of Epstein-Barr Virus Nuclear Antigen 1 in Primary Tumor and Peripheral Blood Cells of Patients with Nasopharyngeal Carcinoma. Clin Cancer Res. 2002 August; 8: 2586–2590.

Skare J, Edson C, Farley J, Strominger J. The B95-8 isolate of Epstein-Barr virus arose from an isolate with a standard genome. J Virol. 1982, December; 44(3): 1088-1091.

Carbone A, Cesarman E, Spina M, Gloghini A, Schulz T. HIV-associated lymphomas and gamma-herpesviruses. Blood. 2009, February 5; 113(6): 1213-1224.

Gulley ML. Molecular Diagnosis of Epstein-Barr Virus-Related Diseases. J Mol Diagn. 2001 February; 3(1).

Tumwine LK, Orem J, Kerchan P, Byarugaba W, Pileri SA. EBV,HHV8 and HIV in B cell non Hodgkin lymphoma in Kampala, Uganda. Infect Agent Cancer. 2010 June 30; 5(12): 1-7, doi: 10.1186/1750-9378-5-12.

Hjalgrim H, Askling J, Madsen M, Rosdahl N, Storm HH. Risk of Hodgkin’s Disease and Other Cancers After Infectious Mononucleosis. J Natl Cancer I. 2000 September; 92(18): 1522-8.

Farrell K, Jarrett RF. The molecular pathogenesis of Hodgkin Lymphoma. Histopathology. 2011 january; 58(1): 15-25.

Čičkušić E, Mustedanagić-Mujanović J, Iljazović E, Karasalihović Z, Škaljić I. Association of Hodgkin’s Lymphoma with Epstein Barr Virus Infection. Bosn J Basic Med Sci. 2007 February; 7(1): 58-65.

Flavell KJ, Murray PG. Hodgkin’s disease and the Epstein-Barr virus. J Clin Pathol. 2000 October; 53(5): 262-9.

Levin LI, Chang ET, Ambinder RF, Lennette ET, Rubertone MV, Mann RB, et al. Atypical prediagnosis Epstein-Barr virus serology restricted to EBV-positive Hodgkin lymphoma. Blood. 2012 September; 120 (18): 3750-5

Glaser SL, Hsu JL. Hodgkin's disease in Asians: Incidence patterns and risk factors in population-based data. Leukemia Res. 2002; 26(3): 261-9.

Shenoy P, Maggioncalda A, Malik N, Flowers C. Incidence Patterns and Outcomes for Hodgkin Lymphoma Patients in the United States. Adv Hematol2011, doi:10.1155/2011/725219.

Macsween KF, Crawford DH. Epstein-Barr virus—recent advances. Lancet Infect Dis. 2003 March; 3: 131-40.

Bakshi N, Maghfoor I. The Current Lymphoma Classification: New Concepts and Practical Applications—Triumphs and Woes. Ann Saudi Med. 2012 May-June; 32(3): 296-305.

Steidl C, Connors JM, Gascoyne RD. Molecular Pathogenesis of Hodgkin’s Lymphoma: Increasing Evidence of the Importance of the Microenvironment. J Clin Oncol. 2011 April 11, doi: 10.1200/JCO.2010.32.8401.

Najafipour S, Mokhtari Azad T, Kousari F, Mahmoodi M, Murray PG, Nategh R. Association of Epstein-Barr virus and hodgkin disease. Acta Medica Iraninca. 2003; 41(1): 1-10.

Al-Salam S, John A, Daoud S, Chong S, Castella A. Expression of Epstein-Barr virus in Hodgkin lymphoma in a population of United Arab Emirates nationals. Leuk Lymphoma. 2008 September; 49(9): 1769-77.

Barros MH, Hassan R, Niedobitek G. Disease patterns in pediatric classical Hodgkin lymphoma: a report from a developing area in Brazil. Hematol Oncol. 2011 December; 29(4): 190-5.

Diepstra A, Van Imhoff GW, Schaapveld M, Karim-Kos H, van den Berg A, Vellenga E, et al. Latent Epstein-Barr virus infection of tumor cells in classical Hodgkin's lymphoma predicts adverse outcome in older adult patients. J Clin Oncol. 2009 August 10; 27(23): 3815-21.

Zhao P, Lu Y, Liu L, Zhong M. Aberrant cytoplasmic expression of cyclin B1 protein and its correlation with EBV-LMP1, P53 and P16(INK4A) in classical Hodgkin lymphoma in China. Pathol Oncol Res. 2011 June; 17(2): 369-73.

Zhou XG, Hamilton-Dutoit SJ, Yan QH, Pallesen G. The association between Epstein-Barr virus and Chinese Hodgkin's disease. Int J Cancer. 1993 September; 55(3): 359-63.

Zarate-Osorno A, Roman LN, Kingma DW, Meneses-Garcia A, Jaffe ES. Hodgkin's disease in Mexico. Prevalence of Epstein-Barr virus sequences and correlations with histologic subtype. Cancer. 1995 March 15; 75(6): 1360-6.

Beiderbeck AB, Holly EA, Sturkenboom MCJM, Coebergh JWW, Stricker BHC, Leufkens HGM. Prescription Medications Associated with a Decreased Risk of Non-Hodgkin’s Lymphoma. Am J Epidemiol. 2003; 157(6): 510-516.

Basiratnia M, Baradaran-Heravi A, Yavarian M, Geramizadeh B, Karimi M. Non-Hodgkin Lymphoma in a Child with Schimke Immuno-Osseous Dysplasia. Iran J Med Sci. 2011 September; 36(3): 222-5.

Fisher SG, Fisher RI. The epidemiology of non-Hodgkin’s lymphoma. Oncogene. 2004; 23: 6524–34.

Evens AM, Winter JN, Gordon LI, Chiu BC, Tsang R, Rosen ST. CancerNetwork home of the journal oncology. [Online].; 2011 [cited 2013 1 24. Available from: http://www.cancernetwork.com/cancer-management/non-hodgkin lymphoma/article/10165/1802748.

Kasprzak A, Spachacz R, Wachowiak J, Stefañska K, Zabel M. Epstein-Barr virus (EBV) infection in B-cell non-Hodgkin's lymphomas in children: virus latency and its correlation with CD21 and CD23 molecules. Folia Histochem Cyto. 2007; 45(3): 169-179.

Pourakbari B, mamishi S, Pajand O, Naji AR, Mahjoub F, Kouchakzadeh L, et al. Detection of Epstein- Barr virus infection in lymphoma: ELISA and PCR method. Tehran Univ Med J. 2010 February; 67(11): 787-792.

Kosari F, Yarigarravesh HR, Rezvan M. Determining the correlation of Epstein-Barr virus with diffuse large B-cell lymphoma by chromogenic in situ hybridization. Tehran Univ Med J. 2012 September; 70(6): 351-6.

Mitarnun W, Pradutkanchana J, Takao S, Saechan V, Suwiwat S, Ishida T. Epstein-barr virus-associated non-Hodgkin's lymphoma of B-cell origin, Hodgkin's disease, acute leukemia, and systemic lupus erythematosus: a serologic and molecular analysis. J Med Assoc Thai. 2002 May; 85(5): 552-559.

Chabay P, Lara J, Lorenzetti M, Cambra P, Acosta Haab G, Aversa L, et al. Epstein Barr virus in relation to apoptosis markers and patients' outcome in pediatric B-cell non-Hodgkin lymphoma. Cancer Lett. 2011 May 4; 307(2): 221-6.

Bahnassy AA, Zekri ARN, El-Houssini S, Khalid HM, Sedky LM, Mokhtar NM. epstein barr virus in head and neck extranodal non-hodgkin Lymphoma in Egypt. Journal of Egyptian Nat. Cancer Inst. 2003 December; 15(4): 349-62.

Umar CS. New Developments in Epstein-Barr Virus Research. In Umar CS, editor.. New York: Nova Publishers; 2006.

Frappier L. The Epstein-Barr Virus EBNA1 Protein. Scientifica2012: 1-15. Article ID: 438204

Frappier LD. Details about NEW EBNA1 and Epstein-Barr Virus Associated Tumours: Springer publisher, 2013. http://link.springer.com/book/10.1007%2F978-1-4614-6886-8.

Robaina TF, Valladares CP, Tavares DS, Napolitano WC, Silva LE, Dias EP, et al. Polymerase chain reaction genotyping of Epstein-Barr virus in scraping samples of the tongue lateral border in HIV-1 seropositive patients. Mem Inst Oswaldo Cruz, Rio de Janeiro. 2008 June; 103(4): 326-31.


Refbacks

  • There are currently no refbacks.


Creative Commons Attribution-NonCommercial 3.0

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.