Expression Level of MicroRNA-122 in Serum Samples of Patients With Atherosclerosis
-
Samin Aboulhassanzadeh
,
Hamed Aghazadeh
,
Zahra Nikkhooy
,
Zahra Sadat Mousavian Hiagh
,
Behboud Jafari
,
Sobhan Aboulhassanzadeh
Abstract
Atherosclerosis is another type of arteriosclerosis, characterized by the hardening of arteries, playing a significant role in various cardiovascular diseases. It stands as one of the leading causes of mortality in contemporary societies, especially in advanced countries experiencing higher levels of stress. The primary feature of atherosclerosis is the accumulation of excessive lipid deposits (plaques), obstructing blood flow and leading to strokes and various cardiovascular diseases. While various factors contribute to plaque formation, both environmental and genetic factors play crucial roles. In the cross-sectional study, 80 patients with atherosclerosis, referred to Rajaee Hospital in Tehran and Madani Hospital in Tabriz, along with 80 healthy individuals for comparative analysis, were selected. All relevant information and serum levels of important biomarkers were measured, followed by statistical analysis. MicroRNA extraction was performed using Trizol solution, and CDNA was synthesized by adding polyadenine tails to all microRNA samples. The obtained CDNA was utilized for real-time PCR with LNA primers. The results, normalized using Spike RNA, indicated a significant 90% decrease in the serum levels of these microRNAs in patients with atherosclerosis. The findings suggest that microRNA miR-122 could serve as a promising biomarker for diagnosing atherosclerosis.
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24. Vorkas PA, Shalhoub J, Isaac G, Want EJ, Nicholson JK, Holmes E, et al. Metabolic phenotyping of atherosclerotic plaques reveals latent associations between free cholesterol and ceramide metabolism in atherogenesis. J Proteome Res 2015;14:1389-99.
25. Bastien M, Poirier P, Lemieux I, Després JP. Overview of epidemiology and contribution of obesity to cardiovascular disease. Prog Cardiovasc Dis 2014;56:369-81.
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27. Liu J, Li W, Wang S, Wu Y, Li Z, Wang W, et al. MiR‑142‑3p attenuates the migration of CD4+ T cells through regulating actin cytoskeleton via RAC1 and ROCK2 in arteriosclerosis obliterans. PLoS One 2014;9:e95514.
28. Ghasemi R, Vojdanparast M, Hosseinzadeh Maleki M, Yaghubi M. The Effect of the Door to Needle Time of Streptokinase Administration on the QTc Interval and the Incidence of Life-Threatening Arrhythmia in Patients With Anterior Myocardial Infarction. Acta Med Iran 2022;60:144-9.
29. Ghorbani MH, Chaleshi V, Amiri N, Nasserinejad M, Baradaran Ghavami S, Shahrokh S, et al. The Sera miRNA Pattern in Patients Inflammatory Bowel Disease. Acta Med Iran 2024;61:599-606.
2. Mattick JS, Gagen MJ. The evolution of controlled multitasked gene networks: the role of introns and other noncoding RNAs in the development of complex organisms. Mol Biol Evol 2001;18:1611-30.
3. Harrison D, Griendling KK, Landmesser U, Hornig B, Drexler H. Role of oxidative stress in atherosclerosis. Am J Cardiol 2003;91:7A-11A.
4. Coffey AR, Smallwood TL, Albright J, Hua K, Kanke M, Pomp D, et al. Systems genetics identifies a co-regulated module of liver microRNAs associated with plasma LDL cholesterol in murine diet-induced dyslipidemia. Physiol Genomics 2017;49:618-29.
5. Fawzy H, Saad N, Elbanna KA, Said HEM, Helal RY, Sameh H. Plasma levels of miR‑29a and miR‑146a in relation to carotid intima media thickness measurement for detection of carotid atherosclerosis. J Med Pharm Chem Res 2024;6:1496-511.
6. Fawzy H, Hendawy D, Ghareeb M, Amer M, Sameh H, Mosaad H. The value of plasma miR‑126 and miR‑423‑3p levels in prediction of subclinical atherosclerotic coronary artery disease. J Med Pharm Chem Res 2024;6:609-22.
7. Taher HR, Saifalla PH. Study of the level of signal‑regulated kinase 5 (ERK5) in patients with coronary heart disease with and without diabetes mellitus type 2. J Med Pharm Chem Res 2023;5:425-40.
8. Ali AA, Al‑Khafaji AH, Al Bayati M, Kuznetsov O, Mohammad W, Saleh M. Role of calcium/potassium coefficient in calcium‑regulating hormone activity in pre‑obese patients. J Med Pharm Chem Res 2023;5:144-53.
9. Abbas Al‑Ameri M, Al‑Rubaei Z. Vascular endothelial growth factor‑A (VEGF‑A) and its receptor (VEGFR‑2) in rheumatoid arthritis patients with type 2 diabetes mellitus. J Med Pharm Chem Res 2022;4:1201-8.
10. Ali ES, Abdul‑Razaq A. Evaluation of lipid profile in Iraqi patients with rheumatoid arthritis. J Med Pharm Chem Res 2022;4:1022-5.
11. Hussein G, Al‑Janabi LM, Hussein NY. Study of the participation of lipoprotein lipase gene polymorphism in coronary artery disease. J Med Pharm Chem Res 2022;4:590-7.
12. Al‑Shakarchi W, Abdulaziz N, Mustafa Y. A review of the chemical, pharmacokinetic, and pharmacological aspects of quercetin. J Med Pharm Chem Res 2022;4:645-56.
13. Maleki Dizaj S, Sharifi S, Shahi S, Montazersaheb S, Salatin S, Ahmadian E, et al. The most important consideration in clinical usage of curcumin. J Med Pharm Chem Res 2022;4:1224-136.
14. Abd‑Alhussain S, Mohammed S. Association of osteopontin with thyroid dysfunction in type II diabetes mellitus. J Med Pharm Chem Res 2021;3:806-11.
15. Harker LA, Ross R, Slichter SJ, Scott CR. Homocystine‑induced arteriosclerosis: the role of endothelial cell injury and platelet response in its genesis. J Clin Invest 1976;58:731-41.
16. Katare R, Riu F, Mitchell K, Gubernator M, Campagnolo P, Cui Y, et al. Transplantation of human pericyte progenitor cells improves the repair of infarcted heart through activation of an angiogenic program involving micro‑RNA‑132. Circ Res 2011;109:894-906.
17. Urbich C, Kuehbacher A, Dimmeler S. Role of microRNAs in vascular diseases, inflammation, and angiogenesis. Cardiovasc Res 2008;79:581-8.
18. Kuo HC, Hsieh KS, Guo MM, Weng KP, Ger LP, Chan WC, et al. Next‑generation sequencing identifies microRNA‑based biomarker panel for Kawasaki disease. J Allergy Clin Immunol 2016;138:1227-30.
19. Hollis M, Nair K, Vyas A, Chaturvedi LS, Gambhir S, Vyas D. MicroRNAs potential utility in colon cancer: early detection, prognosis, and chemosensitivity. World J Gastroenterol 2015;21:8284-92.
20. Kannel WB, Castelli WP, Gordon T, McNamara PM. Serum cholesterol, lipoproteins, and the risk of coronary heart disease: the Framingham study. Ann Intern Med 1971;74:1-12.
21. McGill HC Jr, McMahan CA, Herderick EE, Zieske AW, Malcom GT, Tracy RE, et al. Obesity accelerates the progression of coronary atherosclerosis in young men. Circulation 2002;105:2712-8.
22. Libby P, Ridker PM, Maseri A. Inflammation and atherosclerosis. Circulation 2002;105:1135-43.
23. Badimon JJ, Badimon L, Fuster V. Regression of atherosclerotic lesions by high density lipoprotein plasma fraction in the cholesterol‑fed rabbit. J Clin Invest 1990;85:1234-41.
24. Vorkas PA, Shalhoub J, Isaac G, Want EJ, Nicholson JK, Holmes E, et al. Metabolic phenotyping of atherosclerotic plaques reveals latent associations between free cholesterol and ceramide metabolism in atherogenesis. J Proteome Res 2015;14:1389-99.
25. Bastien M, Poirier P, Lemieux I, Després JP. Overview of epidemiology and contribution of obesity to cardiovascular disease. Prog Cardiovasc Dis 2014;56:369-81.
26. Hamed‑Berair RE, Sithu SD, Wickramasinghe N, Shah J, Agawral A, Winner MG, et al. Role of microRNA‑21 in atherosclerosis. Arterioscler Thromb Vasc Biol 2016;36:A263.
27. Liu J, Li W, Wang S, Wu Y, Li Z, Wang W, et al. MiR‑142‑3p attenuates the migration of CD4+ T cells through regulating actin cytoskeleton via RAC1 and ROCK2 in arteriosclerosis obliterans. PLoS One 2014;9:e95514.
28. Ghasemi R, Vojdanparast M, Hosseinzadeh Maleki M, Yaghubi M. The Effect of the Door to Needle Time of Streptokinase Administration on the QTc Interval and the Incidence of Life-Threatening Arrhythmia in Patients With Anterior Myocardial Infarction. Acta Med Iran 2022;60:144-9.
29. Ghorbani MH, Chaleshi V, Amiri N, Nasserinejad M, Baradaran Ghavami S, Shahrokh S, et al. The Sera miRNA Pattern in Patients Inflammatory Bowel Disease. Acta Med Iran 2024;61:599-606.
| Files | ||
| Issue | Vol 64 No 4 (2026) | |
| Section | Original Articles | |
| Keywords | ||
| Atherosclerosis miR-122 Spike RNA Expression level PCR | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Aboulhassanzadeh S, Aghazadeh H, Nikkhooy Z, Mousavian Hiagh ZS, Jafari B, Aboulhassanzadeh S. Expression Level of MicroRNA-122 in Serum Samples of Patients With Atherosclerosis. Acta Med Iran. 2026;64(4):183-189.
