Clinical Grade Human Adipose Tissue-Derived Mesenchymal Stem Cell Banking
-
Bagher Larijani
,
Hamidreza Aghayan
,
Parisa Goodarzi
,
Fereshteh Mohamadi-Jahani
,
Abbas Norouzi-Javidan
,
Ahmad Reza Dehpour
,
Khadijeh Fallahzadeh
,
Forough Azam Sayahpour
,
Kazem Bidaki
,
Babak Arjmand
Abstract
In this study, our aim was to produce a generation of GMP-grade adipose tissue-derived mesenchymal stem cells for clinical applications. According to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cGMP) compliant adipose tissue-derived mesenchymal stem cells from five female donors. The isolated cells were cultured in DMEM supplemented with 10% fetal bovine serum and characterized by standard methods. Moreover, karyotyping was performed to evaluate chromosomal stability. Mean of donors’ age was 47.6 ± 8.29 year, mean of cell viability was 95.6 ± 1.51%, and cell count was between 9×106 and 14×106 per microliter with the mean of 12.2×106 ± 2863564.21 per microliter. The main aim of this project was demonstrating the feasibility of cGMP-compliant and clinical grade adipose tissue-derived mesenchymal stem cells preparation and banking for clinical cell transplantation trials.
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| Files | ||
| Issue | Vol 53, No 9 (2015) | |
| Section | Original Articles | |
| Keywords | ||
| Adipose-derived stem cell Cell therapy Good manufacturing practice Mesenchymal stem cells | ||
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