The Effect of Low Omega-3/Omega-6 Ratio on Auditory Nerve Conduction in Rat Pups

  • Saeid Farahani Department of Audiology , School of Rehabilitation, Tehran University of Medical Sciences,Tehran,Iran.
  • Masoud Motasaddi Zarandy Mail Department of Otolaryngology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. AND ENT Research Center, Tehran University of Medical Sciences, Tehran, Iran.
  • Gholamreza Hassanzadeh Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Farzad Shidfar School of Health, Iran University of Medical Sciences, Tehran, Iran.
  • Shohreh Jalaie Department of Biostatistics, School of Rehabilitation, Tehran University of Medical Sciences, Tehran, Iran.
  • Vida Rahimi Department of Audiology, School of Rehabilitation, Tehran University of Medical Sciences, Tehran, Iran.
Omega-3 omega-6 ratio, Auditory nerve conduction, Rat pups


The biological effects of omega-3 and omega-6 fatty acids are determined by their mutual interactions. This interaction extremely affects various functions. Lower consumption of omega-3 during gestation leads to various disorders, even in hearing. We aimed to assess the effect of low omega-3/omega-6 ratios on auditory nerve conduction. In this experimental study, the auditory brainstem response test was performed on 24-day-old rat (n=14). The rats were divided into case (low omega-3/omega-6 ratio during gestation and lactation) and control groups. Variables such as P1, P3, and P4 absolute latency period, interpeaks (P3-P4, P1-P3, and P1-P4), and P4/P1 amplitude ratio were measured. We found an increased P4 omega-3/omega-6 ratio in the group with a low omega-3/omega-6 ratio (P<0.01). No significant difference was observed in the P1 and P3 absolute latency period between the studied groups  (P>0.05).  Also, no significant difference was observed between the groups with respect to the P1-P3 interpeak latency (IPL) periods (P>0.05); while the P1-P4 and P3-P4 IPLs were significantly increased in the group with a low omega-3/omega-6 ratio (P<0.05). The P4/P1 amplitude ratio significantly decreased in the group with a low omega-3/omega-6 ratio (P<0.05). Results confirmed the negative effects of low omega-3/omega-6 ratio on the auditory system and hearing.


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How to Cite
Farahani S, Motasaddi Zarandy M, Hassanzadeh G, Shidfar F, Jalaie S, Rahimi V. The Effect of Low Omega-3/Omega-6 Ratio on Auditory Nerve Conduction in Rat Pups. Acta Med Iran. 53(6):346-350.