The Effect of Low Omega-3/Omega-6 Ratio on Auditory Nerve Conduction in Rat Pups
-
Saeid Farahani
,
Masoud Motasaddi Zarandy
,
Gholamreza Hassanzadeh
,
Farzad Shidfar
,
Shohreh Jalaie
,
Vida Rahimi
Abstract
The biological effects of omega-3 and omega-6 fatty acids are determined by their mutual interactions. This interaction extremely affects various functions. Lower consumption of omega-3 during gestation leads to various disorders, even in hearing. We aimed to assess the effect of low omega-3/omega-6 ratios on auditory nerve conduction. In this experimental study, the auditory brainstem response test was performed on 24-day-old rat (n=14). The rats were divided into case (low omega-3/omega-6 ratio during gestation and lactation) and control groups. Variables such as P1, P3, and P4 absolute latency period, interpeaks (P3-P4, P1-P3, and P1-P4), and P4/P1 amplitude ratio were measured. We found an increased P4 omega-3/omega-6 ratio in the group with a low omega-3/omega-6 ratio (P<0.01). No significant difference was observed in the P1 and P3 absolute latency period between the studied groups (P>0.05). Also, no significant difference was observed between the groups with respect to the P1-P3 interpeak latency (IPL) periods (P>0.05); while the P1-P4 and P3-P4 IPLs were significantly increased in the group with a low omega-3/omega-6 ratio (P<0.05). The P4/P1 amplitude ratio significantly decreased in the group with a low omega-3/omega-6 ratio (P<0.05). Results confirmed the negative effects of low omega-3/omega-6 ratio on the auditory system and hearing.
Stillwell W, Shaikh SR, Zerouga M. Docosahexaenoic acid affects cell signaling by altering lipid rafts. Reprod Nutr Dev 2005;45(5):559-79.
Calder PC. n-3 fatty acids, inflammation, and immunityrelevance to postsurgical and critically ill patients. Lipids 2004;39(12):1147-61.
Indu M, Ghafoorunissa. n-3 fatty acids in Indian diets comparison of the effects of precursor (alpha-linolenic acid) vs product (long chain n-3 polyunsaturated fatty acids). Nutr Res1992;12(4-5):569-82.
Rahimi V, Farahani S, Nobakht M, et al. Effect of omega-3 on the auditory system. Audiol 2013;22(4):1-15.
Singh M. Essential Fatty Acids, DHA and the Human Brain. Indian J Pediatr 2005;72(3):239-42.
Haubner L, Sullivan J, Ashmeade T, et al. The effects of maternal dietary docosahexaenoic acid intake on rat pup myelin and the auditory startle response .Dev Neurosci 2007;29(6):460-7.
Crawford MA, Golfetto I, Ghebremeskel K. The potentialrole for arachidonic and docosahexaenoicacids in protection against some central nervous system injuries in preterm infants. Lipids 2003;38(4):303-15.
Simopoulos AP. The importance of the ratio of omega- 6/omega-3 essential fatty acids. Biomed Pharmacother 2002;56(8):365-79.
Morse NL. A meta-analysis of blood fatty acids in people with learning disorders with particular interest in arachidonicacid: Prostaglandins, Leukotrienes and Essential Fatty Acids. Prostaglandins Leukot Essent Fatty Acids 2009;81(5-6):373-89.
Haubner L.Y, Stockard J.E, Saste M.D. Maternal dietary docosahexanoic acid content affects the rat pup auditory system. Brain Res. Bull 2002;58(1):1-5.
Pourbakht A, Imani A. The Protective Effect of Conditioning on Noise-Induced Hearing Loss Is Frequency-Dependent. Acta Med Iran 2012;50(10):664-9.
Church MW, Jen KL, Stafferton T, et al. Reduced Auditory Acuity in Rat Pups from Excess and Deficient Omega-3 Fatty Acid Consumption by the Mother. Neurotoxicol Teratol 2007;29(2):203-10.
Bourre JM, Durand G, Erre JP, et al. Changes in auditory brainstem responses in alpha-linolenic acid deficiency as a function of age in rats. Audiology 1999;38(1):13-8.
Church MW, Jen KL, Jackson DA, et al. Abnormal neurological responses in young adult offspring caused by excess omega-3 fatty acid (fish oil) consumption by the mother during pregnancy and lactation. Neurotoxicol Teratol 2009;31(1):26-33.
Stockard JE, Saste MD, Benford VJ, et al. Effect of docosahexaenoic acid content of maternal diet on auditory brainstem conduction times in rat pups. Dev Neurosci 2000;22(5-6):494-9.
Church MW, Blakley BW, Burgio DL, et al. WR-2721 (Amifostine) ameliorates cisplatin-induced hearing loss but causes neurotoxicity in hamsters: dose-dependent effects. J Assoc Res Otolaryngol 2004;5(3):227-37.
Henry KR. Auditory brainstem volume-conducted responses: origins in the laboratory mouse. J Am Aud Soc 1979;4(5):173-8.
Church MW, Jen KL, Anumba JI. Excess omega-3 fatty acid consumption by mothers during pregnancy and lactation caused shorter life span and abnormal ABRs in old adult offspring. Neurotoxicol Teratol 2010;32(2):171-81.
Church MW, Jen KL, Dowhan LM, et al. Excess and deficient omega-3 fatty acid during pregnancy and lactation cause impaired neural transmission in rat pups. Neurotoxicol Teratol 2008;30(2):107-17.
Alvarado JC, Fuentes-Santamaría V, Jareño-Flores T, et al. Normal variations in the morphology of auditory brainstem response (ABR) waveforms: a study in Wistar rats. Neurosci Res 2012;73(4):302-11.
de la Presa Owens S, Innis SM. Diverse, region-specific effects of addition ofarachidonic and docosahexanoic acids to formula with low or adequate linoleic andalphalinolenic acids on piglet brain monoaminergic neurotransmitters. Pediatr Res 2000;48(1):125-30.
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| Issue | Vol 53, No 6 (2015) | |
| Section | Articles | |
| Keywords | ||
| Omega-3 omega-6 ratio Auditory nerve conduction Rat pups | ||
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