Articles

Association Between Expression of Interleukin-32 Gene and Various Helicobacter pylori Virulence Factors in Human Infected Gastric Biopsy

Abstract

Helicobacter pylori (H. pylori) is a spiral bacterium that infects the human gastric mucosa. Various clinical aspects of the infection may mirror distinctive forms of cytokine expression. It has a correlation with immune cell penetration to the gastric mucosa with numerous cytokines production and gastric inflammation. IL-1 and IL-8 are directly contribute to H. pylori effected gastritis. IL-32 is a pro-inflammatory cytokine categorized by the training of Immune cells activation which have a vital role in human immunity. H. pylori virulence and danger factors which are critical in gastritis such as the outer inflammatory protein (OipA) and the cytotoxin associated gene A (cagA). We aimed to study the IL-32 mRNA expression in H. pylori-positive and negative patients as well as its relation with bacterial cagA, oipA, and severity of gastritis. Endoscopic biopsies were taken from the antrum of 60 H. pylori-infected patients and 62 uninfected individuals.  Mucosal IL-32 mRNA expression was assessed by real-time PCR. With PCR the H. pylori virulence factors were evaluated. Showed that the mRNA expression of IL-32 levels were significantly lower in biopsies of H. pylori-uninfected patients compared to positive individual (p = 0.01). A straight communication between virulence factor oipA, cagA and heightening in IL-32 mRNA expression (p <0.001) was observed. Furthermore. IL-32 mRNA expression levels were approximately equal in both chronic and active gastritis (p = 0.1). IL-32 may have critical role in different situation like inflammation and severity of inflammatory changes in the gastric mucosa.

genotype in Helicobacter pylori from Patient with Gastroduodenal Diseases in firouzgar hospital tehran. Goversh 2012;17:78-83.
18. Mun SH, Jie Wan Kim, Seong Su Nah, Na Young Ko, Jun Ho Lee, Ju Dong Kim. Tumor necrosis factor alpha-induced interleukin-32 is positively regulated via the Syk/protein kinase C delta/JNK pathway in rheumatoid synovial fibroblasts. Arthritis Rheum 2009;60:678-85.
19. Heinhuis B, Marije I Koenders, Fons A van de Loo, Mihai G Netea, Wim B van den Berg, Leo A B Joosten. Inflammation-dependent secretion and splicing of IL-32 gamma in rheumatoid arthritis. Proc Natl Acad Sci USA 2011;108:4962-67.
20. Alsaleh G, Sparsa L, Chatelus E, Ehlinger M, Gottenberg J-E, Wachsmann D, et al. Innate immunity triggers IL-32 expression by fibroblast-like synoviocytes in rheumatoid arthritis. Arthritis Res Ther 2010;12:135.
21. Sakitani K, Hirata Y, Hayakawa Y, Serizawa T, Nakata W, Takahashiet R, et al. Role of interleukin-32 in Helicobacter pylori-induced gastric inflammation. Infect Immun 2012; 80:3795-803.
22. Meyer N, Zimmermann M, Bürgler S, Bassin C, Woehrl S, Moritz K, et al. IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis. J Allergy Clin Immunol 2010;125:858-65.
23. Carmi Y, Rinott G, Dotan SH, Elkabets M, Rider P, Voronov E, et al. Microenvironment-derived IL-1 and IL-17 interact in the control of lung metastasis. J Immunol 2011;186:3462-71.
24. Ishigami S, Arigami T, Uchikado Y, Setoyama T, Kita Y,
Sasaki K, et al. IL-32 expression is an independent prognostic marker for gastric cancer. Med Oncol 2013;30:472.
25. Sorrentino C, Di Carlo E. Expression of IL-32 in human lung cancer is related to the histotype and metastatic phenotype. Am J Respir Crit Care Med 2009;180:769-79.
26. Fukase K, Kato M, Kikuchi SH, Inoue K, Uemura N, and Okamoto SH, et al. Effect of eradication of Helicobacter pylori on incidence of metachronous gastric carcinoma after endoscopic resection of early gastric cancer: an open-label, randomised controlled trial. Lancet 2008;372:392-97.
27. Hirata Y, Maeda SH, Ohmae T, Shibata W, Yanai A, Ogura K. Helicobacter pylori induces I kappa B kinase at nuclear translocation and chemokine production in gastric epithelial cells. Infect. Immun 2006; 74:1452-61.
28. Kim S.H, Han S Y, Azam T, Yoon D Y, Dinarello CH A. Interleukin-32: a cytokine and inducer of TNFalpha. Immunity 2005; 22:131-42.
29. Netea M.G, Azam T, Ferwerda G, Girardin S E, Walsh M, Park J S, et al., IL-32 synergizes with nucleotide oligomerization domain (NOD) 1 and NOD2 ligands for IL-1beta and IL-6 production through a caspase 1-dependent mechanism. Proc Natl Acad Sci U S A 2005,102:16309-14.
30. Shafika A, Costanian CH, Jaffal L, Tannous F, Stathopoulou M G, and Shamieh S E. Christy Costanian, Lama Jaffal, et al. Association of TLR4 Polymorphisms, Expression, and Vitamin D with Helicobacter pylori Infection , et al. J Pers Med 2019;9:2.
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IssueVol 58, No 3 (2020) QRcode
SectionArticles
DOI https://doi.org/10.18502/acta.v58i3.3772
Keywords
Interleukin-32 Helicobacter pylori Virulence factors Gastritis
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How to Cite
1.
Shahi H, Kiaee F. Association Between Expression of Interleukin-32 Gene and Various Helicobacter pylori Virulence Factors in Human Infected Gastric Biopsy. Acta Med Iran. 2020;58(3):103-108.